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bacteria:t3e:xopn [2020/08/09 16:31] jvicente |
bacteria:t3e:xopn [2020/08/09 20:32] (current) jvicente |
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=== How discovered? === | === How discovered? === | ||
- | XopN was identified in a genetic screen, using a Tn// | + | XopN was identified in a genetic screen, using a Tn// |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
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=== Regulation === | === Regulation === | ||
- | Start codon of //xopN// was found downstream | + | Start codon of //xopN// was found downstream |
qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//) were significantly reduced in the // | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//) were significantly reduced in the // | ||
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* Its homolog XopN < | * Its homolog XopN < | ||
* XopN has been shown to play a role in host defence systems causing the reduction of PAMP-triggered immune responses and reduce the callose deposition in the host tissue. Moreover the deletion of // | * XopN has been shown to play a role in host defence systems causing the reduction of PAMP-triggered immune responses and reduce the callose deposition in the host tissue. Moreover the deletion of // | ||
- | * XopN has been shown to be required for maximal pathogenicity of //X. axonopodis// | + | |
+ | | ||
* A Δ// | * A Δ// | ||
- | * XopN was shown to contribute significantly to //X. oryzae// | + | * // |
+ | * XopN and AvrBS2 were shown to significantly contribute to virulence of //X. oryzae// | ||
=== Localization === | === Localization === | ||
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=== Enzymatic function === | === Enzymatic function === | ||
- | Unknown – Kim //et al//. (2009) did not confirm | + | XopN binds TARK1, a tomato atypical receptor kinase required for PTI. Taylor |
+ | |||
+ | Three effectors (XopZ, XopN and XopV) were shown to be able to supress the peptidoglycan-triggered MAPK activation and a triple mutant of Xoo lacking these genes showed additively reduced virulence (Long et al., 2018). | ||
=== Interaction partners === | === Interaction partners === | ||
- | XopN interact with two types of proteins in tomato: Tomato Atypical Receptor-like Kinase1 (TARK1) and four Tomato Fourteen-Three-Three isoforms (TFT1, TFT3, TFT5, and TFT6) (Kim //et al//., 2009). | + | XopN interact with two types of proteins in tomato: Tomato Atypical Receptor-like Kinase1 (TARK1) and four Tomato Fourteen-Three-Three isoforms (TFT1, TFT3, TFT5, and TFT6) (Kim //et al//., 2009). XopN interacts with the tomato 14-3-3 isoform TFT1 that functions in PTI and is a XopN virulence target (Taylor //et al.//, 2012). |
+ | |||
+ | Two rice proteins, OsVOZ2 and a putative thiamine synthase (OsXNP) were identified as targets of XopN< | ||
===== Conservation ===== | ===== Conservation ===== | ||
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=== In xanthomonads === | === In xanthomonads === | ||
- | Yes (//e.g.//, //X. axonopodis//, | + | Yes (//e.g.//, //X. axonopodis//, |
=== In other plant pathogens/ | === In other plant pathogens/ | ||
Line 57: | Line 63: | ||
Cheong H, Kim CY, Jeon JS, Lee BM, Sun Moon J, Hwang I (2013). // | Cheong H, Kim CY, Jeon JS, Lee BM, Sun Moon J, Hwang I (2013). // | ||
+ | |||
+ | Dubrow Z, Sunitha S, Kim JG, et al. Tomato 14-3-3 Proteins Are Required for Xv3 Disease Resistance and Interact with a Subset of Xanthomonas euvesicatoria Effectors. //Mol Plant Microbe Interact//. 2018; | ||
+ | |||
+ | Guzman AR, Kim JG, Taylor KW, Lanver D, Mudgett MB. Tomato Atypical Receptor Kinase1 Is Involved in the Regulation of Preinvasion Defense. //Plant Physiol//. 2020; | ||
Jiang B, He Y, Cen W, Wei H, Jiang G, Jiang W, Hang X, Feng J, Lu G, Tang D, Tang J (2008). The type III secretion effector XopXccN of // | Jiang B, He Y, Cen W, Wei H, Jiang G, Jiang W, Hang X, Feng J, Lu G, Tang D, Tang J (2008). The type III secretion effector XopXccN of // | ||
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Kumar R, Mondal KK (2013). XopN-T3SS effector modulates in planta growth of Xanthomonas axonopodis pv. punicae and cell-wall-associated immune response to induce bacterial blight in pomegranate. Physiological and Mol. Plant Pathol. 84: 36-43. DOI: 10.1016/ | Kumar R, Mondal KK (2013). XopN-T3SS effector modulates in planta growth of Xanthomonas axonopodis pv. punicae and cell-wall-associated immune response to induce bacterial blight in pomegranate. Physiological and Mol. Plant Pathol. 84: 36-43. DOI: 10.1016/ | ||
- | Kumar R, Soni M, Mondal KK (2016). XopN-T3SS effector of // | + | Kumar R, Soni M, Mondal KK (2016). XopN-T3SS effector of // |
- | Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in //Xanthomonas oryzae// | + | Liao ZX, Li JY, Mo XY, et al. Type III effectors xopN and avrBS2 contribute to the virulence of Xanthomonas oryzae pv. oryzicola strain GX01. //Res Microbiol//. 2020;171(2):102-106. DOI:10.1016/j.resmic.2019.10.002 |
Liu Y, Long J, Shen D, Song C (2016). // | Liu Y, Long J, Shen D, Song C (2016). // | ||
- | Long J, Song C, Yan F, Zhou J, Zhou H, Yang B (2018). Non-TAL effectors from // | + | Long J, Song C, Yan F, Zhou J, Zhou H, Yang B (2018). Non-TAL effectors from // |
Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // | Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // | ||
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Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // | Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // | ||
- | Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of // | + | Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of // |
- | Taylor KW, Kim JG, Su XB, Aakre CD, Roden JA, Adams CM, Mudgett MB (2012). Tomato TFT1 is required for PAMP-triggered immunity and mutations that prevent T3S effector XopN from binding to TFT1 attenuate // | + | Taylor KW, Kim JG, Su XB, Aakre CD, Roden JA, Adams CM, Mudgett MB (2012). Tomato TFT1 is required for PAMP-triggered immunity and mutations that prevent T3S effector XopN from binding to TFT1 attenuate // |