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bacteria:t3e:xopn [2020/08/09 19:54] jvicente |
bacteria:t3e:xopn [2020/08/09 20:32] (current) jvicente |
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=== How discovered? === | === How discovered? === | ||
- | XopN was identified in a genetic screen, using a Tn// | + | XopN was identified in a genetic screen, using a Tn// |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
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=== Regulation === | === Regulation === | ||
- | Start codon of //xopN// was found downstream of a conserved cis-regulatory element, the plant-inducible promoter (PIP) box (TTCGG-N15-TTCTG). //xopN// is regulated by //hrpX// and //hrpG// genes (Cheong | + | Start codon of //xopN// was found downstream of a conserved cis-regulatory element, the plant-inducible promoter (PIP) box (TTCGG-N15-TTCTG). //xopN// is regulated by //hrpX// and //hrpG// genes (Jiang //et al// |
qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//) were significantly reduced in the // | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//) were significantly reduced in the // | ||
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* XopN has been shown to play a role in host defence systems causing the reduction of PAMP-triggered immune responses and reduce the callose deposition in the host tissue. Moreover the deletion of // | * XopN has been shown to play a role in host defence systems causing the reduction of PAMP-triggered immune responses and reduce the callose deposition in the host tissue. Moreover the deletion of // | ||
* The role of XopN in X. oryzae pv. oryzae is dependent on leaf stage (Cheong et al., 2013). | * The role of XopN in X. oryzae pv. oryzae is dependent on leaf stage (Cheong et al., 2013). | ||
- | * XopN has been shown to be required for maximal pathogenicity of //X. axonopodis// | + | * XopN has been shown to be required for maximal pathogenicity of //X. axonopodis// |
* A Δ// | * A Δ// | ||
- | * XopN was shown to contribute significantly to //X. oryzae// | + | * // |
+ | * XopN and AvrBS2 were shown to significantly contribute to virulence of //X. oryzae// | ||
=== Localization === | === Localization === | ||
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=== Enzymatic function === | === Enzymatic function === | ||
- | XopN binds TARK1, a tomato atypical receptor kinase required for PTI. Taylor //et al.// (2012) showed that XopN promotes TARK1/TFT1 complex formation | + | XopN binds TARK1, a tomato atypical receptor kinase required for PTI. Taylor //et al.// (2012) showed that XopN promotes TARK1/TFT1 complex formation |
+ | |||
+ | Three effectors (XopZ, XopN and XopV) were shown to be able to supress the peptidoglycan-triggered MAPK activation and a triple mutant of Xoo lacking these genes showed additively reduced virulence (Long et al., 2018). | ||
=== Interaction partners === | === Interaction partners === | ||
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XopN interact with two types of proteins in tomato: Tomato Atypical Receptor-like Kinase1 (TARK1) and four Tomato Fourteen-Three-Three isoforms (TFT1, TFT3, TFT5, and TFT6) (Kim //et al//., 2009). XopN interacts with the tomato 14-3-3 isoform TFT1 that functions in PTI and is a XopN virulence target (Taylor //et al.//, 2012). | XopN interact with two types of proteins in tomato: Tomato Atypical Receptor-like Kinase1 (TARK1) and four Tomato Fourteen-Three-Three isoforms (TFT1, TFT3, TFT5, and TFT6) (Kim //et al//., 2009). XopN interacts with the tomato 14-3-3 isoform TFT1 that functions in PTI and is a XopN virulence target (Taylor //et al.//, 2012). | ||
- | Two rice proteins, OsVOZ2 and a putative thiamine synthase (OsXNP) were identified as targets of XopN< | + | Two rice proteins, OsVOZ2 and a putative thiamine synthase (OsXNP) were identified as targets of XopN< |
===== Conservation ===== | ===== Conservation ===== | ||
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Dubrow Z, Sunitha S, Kim JG, et al. Tomato 14-3-3 Proteins Are Required for Xv3 Disease Resistance and Interact with a Subset of Xanthomonas euvesicatoria Effectors. //Mol Plant Microbe Interact//. 2018; | Dubrow Z, Sunitha S, Kim JG, et al. Tomato 14-3-3 Proteins Are Required for Xv3 Disease Resistance and Interact with a Subset of Xanthomonas euvesicatoria Effectors. //Mol Plant Microbe Interact//. 2018; | ||
- | Guzman AR, Kim JG, Taylor KW, Lanver D, Mudgett MB. Tomato Atypical Receptor Kinase1 Is Involved in the Regulation of Preinvasion Defense. //Plant Physiol//. 2020; | + | Guzman AR, Kim JG, Taylor KW, Lanver D, Mudgett MB. Tomato Atypical Receptor Kinase1 Is Involved in the Regulation of Preinvasion Defense. //Plant Physiol//. 2020; |
Jiang B, He Y, Cen W, Wei H, Jiang G, Jiang W, Hang X, Feng J, Lu G, Tang D, Tang J (2008). The type III secretion effector XopXccN of // | Jiang B, He Y, Cen W, Wei H, Jiang G, Jiang W, Hang X, Feng J, Lu G, Tang D, Tang J (2008). The type III secretion effector XopXccN of // | ||
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Liao ZX, Li JY, Mo XY, et al. Type III effectors xopN and avrBS2 contribute to the virulence of Xanthomonas oryzae pv. oryzicola strain GX01. //Res Microbiol// | Liao ZX, Li JY, Mo XY, et al. Type III effectors xopN and avrBS2 contribute to the virulence of Xanthomonas oryzae pv. oryzicola strain GX01. //Res Microbiol// | ||
- | |||
- | Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in // | ||
Liu Y, Long J, Shen D, Song C (2016). // | Liu Y, Long J, Shen D, Song C (2016). // | ||
- | Long J, Song C, Yan F, Zhou J, Zhou H, Yang B (2018). Non-TAL effectors from // | + | Long J, Song C, Yan F, Zhou J, Zhou H, Yang B (2018). Non-TAL effectors from // |
Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // | Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // | ||
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Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // | Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // | ||
- | Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of // | + | Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of // |
Taylor KW, Kim JG, Su XB, Aakre CD, Roden JA, Adams CM, Mudgett MB (2012). Tomato TFT1 is required for PAMP-triggered immunity and mutations that prevent T3S effector XopN from binding to TFT1 attenuate // | Taylor KW, Kim JG, Su XB, Aakre CD, Roden JA, Adams CM, Mudgett MB (2012). Tomato TFT1 is required for PAMP-triggered immunity and mutations that prevent T3S effector XopN from binding to TFT1 attenuate // | ||