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bacteria:t3e:xopo [2020/04/24 22:40] 127.0.0.1 external edit |
bacteria:t3e:xopo [2020/11/26 16:16] (current) zdubrow |
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Author: Harrold van den Burg\\ | Author: Harrold van den Burg\\ | ||
- | Internal reviewer: | + | Internal reviewer: |
- | Expert reviewer: | + | Expert reviewer: |
Class: XopO\\ | Class: XopO\\ | ||
Family: XopO\\ | Family: XopO\\ | ||
- | Prototype: XopO (// | + | Prototype: XopO (// |
RefSeq ID: [[https:// | RefSeq ID: [[https:// | ||
3D structure: Unknown | 3D structure: Unknown | ||
Line 14: | Line 14: | ||
=== How discovered? === | === How discovered? === | ||
- | XopO was discovered by a random transponson insertion (Tn5) screen using a AvrBs2< | ||
+ | XopO was identified in a genetic screen, using a Tn// | ||
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | XopO fused to the Cya reporter was used to show that it is translocated into plant cells in a // | ||
+ | Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004). | ||
=== Regulation === | === Regulation === | ||
- | XopO was found to be regulated by HrpG using HrpG* (Roden //et al//., 2004). //XopO// contains a PIP box sequence 31bp upstream of the -10 promoter motif (Koebnik //et al//., 2006). | ||
+ | XopO was found to be regulated by HrpG using HrpG* (Roden //et al//., 2004). //XopO// contains a PIP box sequence 31bp upstream of the -10 promoter motif (Koebnik //et al//., 2006). | ||
=== Phenotypes === | === Phenotypes === | ||
- | XopO from Xcv 85-10 inhibits cell death in //N. benthamiana// | + | |
+ | * Roden et al. did not find significant growth defects of a // | ||
+ | * XopO from //Xcv// | ||
+ | * XopO suppresses //X. euvesicatoria-// | ||
+ | * XopO failed to inhibit expression of the reporter gene // | ||
+ | * Based on whole genome sequences of //X. euvesicatoria// | ||
=== Localization === | === Localization === | ||
+ | |||
Unknown. | Unknown. | ||
=== Enzymatic function === | === Enzymatic function === | ||
+ | |||
Unknown. | Unknown. | ||
=== Interaction partners === | === Interaction partners === | ||
- | XopO was shown to interact with tomato 14-3-3- proteins | + | |
+ | XopO was shown to interact with tomato 14-3-3 (TFT) proteins | ||
===== Conservation ===== | ===== Conservation ===== | ||
=== In xanthomonads === | === In xanthomonads === | ||
- | Yes, in some //Xanthomonads// | + | |
+ | Yes, in some xanthomonads (//e.g.//, //X. euvesicatoria//, | ||
=== In other plant pathogens/ | === In other plant pathogens/ | ||
- | Yes (HopK1 from // | + | |
+ | Yes, //e.g.// homologs (AvrRps4 and HopK1) in // | ||
===== References ===== | ===== References ===== | ||
- | Barak JD //et al//. (2016) Whole-genome sequences of // | + | Barak JD, Vancheva T, Lefeuvre P, Jones JB, Timilsina S, Minsavage GV, Vallad GE, Koebnik R (2016) Whole-genome sequences of // |
+ | |||
+ | Dubrow Z, Sunitha S, Kim JG, Aakre CD, Girija AM, Sobol G, Teper D, Chen YC, Ozbaki-Yagan N, Vance H, Sessa G, Mudgett MB (2018). Tomato 14-3-3 proteins are required for // | ||
+ | |||
+ | Hajri A, Brin C, Zhao S, David P, Feng JX, Koebnik R, Szurek B, Verdier V, Boureau T, Poussier S (2012). Multilocus sequence analysis and type III effector repertoire mining provide new insights into the evolutionary history and virulence of // | ||
+ | |||
+ | Koebnik R, Krüger A, Thieme F, Urban A, Bonas U (2006). Specific binding of the Xanthomonas campestris pv. vesicatoria AraC-type transcriptional activator HrpX to plant-inducible promoter boxes. J. Bacteriol. 188: 7652-7660. DOI: [[https:// | ||
- | Dubrow Z //et al//. (2018). Tomato 14-3-3 proteins are required for Xv3 disease resistance and interact with a subset | + | Lang JM, Pérez-Quintero AL, Koebnik R, DuCharme E, Sarra S, Doucoure H, Keita I, Ziegle J, Jacobs JM, Oliva R, Koita O, Szurek B, Verdier V, Leach JE (2019). A pathovar |
- | Hajri A //et al//. (2012). Multilocus sequence analysis and type III effector repertoire mining provide new insights into the evolutionary history and virulence of //Xanthomonas oryzae//. Mol. Plant Pathol. 13, 288-302, | + | Li G, Froehlich JE, Elowsky C, Msanne J, Ostosh AC, Zhang C, Awada T, Alfano JR, (2014). Distinct |
- | Koebnik R, Kruger A, Thieme | + | Popov G, Fraiture M, Brunner |
- | Popov G, Fraiture M, Brunner F, Sessa G (2016). Multiple | + | Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate type III effectors translocated into pepper cells during |
- | Roden JA //et al//. (2004). A genetic screen to isolate type III effectors translocated into pepper cells during | + | Sohn KH, Zhang Y, Jones JD (2009). The //Pseudomonas syringae// |
- | Teper D, Sunitha S, Martin GB, Sessa G (2015). Five Xanthomonas type III effectors suppress cell death induced by components of immunity-associated MAP kinase cascades. Plant Signal Behav. 10: e1064573. DOI: [[https:// | + | Teper D, Sunitha S, Martin GB, Sessa G (2015). Five //Xanthomonas// |