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bacteria:t3e:xopp [2020/07/03 15:23] rkoebnik [Biological function] |
bacteria:t3e:xopp [2022/06/23 09:53] rkoebnik [Biological function] |
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Class: XopP\\ | Class: XopP\\ | ||
Family: XopP\\ | Family: XopP\\ | ||
- | Prototype: XopP (// | + | Prototype: XopP (// |
RefSeq ID: [[https:// | RefSeq ID: [[https:// | ||
3D structure: Unknown | 3D structure: Unknown | ||
Line 15: | Line 15: | ||
=== How discovered? === | === How discovered? === | ||
- | By using a domain of AvrBs2 | + | XopP was identified in a genetic screen, |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | Translocate through | + | Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control |
=== Regulation === | === Regulation === | ||
- | // | + | The // |
qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopP//, were significantly reduced in the // | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopP//, were significantly reduced in the // | ||
=== Phenotypes === | === Phenotypes === | ||
- | Roden //et al.// did not find significant growth defects of a //Xcv// Δ//xopP// mutant in susceptible pepper and tomato leaves (Roden et al., 2004). | + | * Roden //et al.// did not find significant growth defects of a // |
+ | * XopQ< | ||
+ | * XopP< | ||
+ | * // | ||
+ | * XopP inhibits the function of the host-plant exocyst complex by direct targeting of Exo70B, a subunit of the exocyst complex, which plays a significant role in plant immunity. XopP interferes with exocyst-dependent exocytosis, and can do this without activating a plant NLR (NOD-like receptor) that guards Exo70B in Arabidopsis. In this way, // | ||
- | XopP< | ||
=== Localization === | === Localization === | ||
- | XopP< | + | XopP< |
=== Enzymatic function === | === Enzymatic function === | ||
Line 38: | Line 42: | ||
=== Interaction partners === | === Interaction partners === | ||
- | XopP< | + | XopP< |
===== Conservation ===== | ===== Conservation ===== | ||
Line 49: | Line 53: | ||
Yes (//e.g.//, //Ralstonia solanacearum// | Yes (//e.g.//, //Ralstonia solanacearum// | ||
===== References ===== | ===== References ===== | ||
+ | |||
+ | Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the // | ||
Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in // | Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in // | ||
Ishikawa K, Yamaguchi K, Sakamoto K, Yoshimura S, Inoue K, Tsuge S, Kojima C, Kawasaki T (2014). Bacterial effector modulation of host E3 ligase activity suppresses PAMP-triggered immunity in rice. Nat. Commun. 5: 5430. DOI: [[https:// | Ishikawa K, Yamaguchi K, Sakamoto K, Yoshimura S, Inoue K, Tsuge S, Kojima C, Kawasaki T (2014). Bacterial effector modulation of host E3 ligase activity suppresses PAMP-triggered immunity in rice. Nat. Commun. 5: 5430. DOI: [[https:// | ||
+ | |||
+ | Jiang W, Jiang B, Xu R, Huang J, Wei H, Jiang GF, Cen WJ, Liu J, Ge YY, Li GH, Su LL, Hang XH, Tang DJ, Lu GT, Feng JX, He YQ, Tang JL (2009). Identification of six type III effector genes with the PIP box in // | ||
Liu Y, Long J, Shen D, Song C (2016). // | Liu Y, Long J, Shen D, Song C (2016). // | ||
+ | |||
+ | Michalopoulou VA, Mermigka G, Kotsaridis K, Mentzelopoulou A, Celie PHN, Moschou PN, Jones JDG, Sarris PF (2022). The host exocyst complex is targeted by a conserved bacterial type-III effector that promotes virulence. Plant Cell, in press. DOI: [[https:// | ||
Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // | Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // | ||