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bacteria:t3e:xopv [2020/07/17 10:43] rkoebnik [Conservation] |
bacteria:t3e:xopv [2021/01/07 06:08] (current) s_tsuge [Biological function] |
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Author: Coline Sciallano\\ | Author: Coline Sciallano\\ | ||
Internal reviewer: [[https:// | Internal reviewer: [[https:// | ||
- | Expert reviewer: | + | Expert reviewer: |
Class: XopV\\ | Class: XopV\\ | ||
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=== How discovered? === | === How discovered? === | ||
- | XopV was discovered via genome-wide screening for effector candidates in //X. oryzae// pv. //oryzae// (//Xoo//) strain MAFF 311018 in accordance with three criteria: i) ORF encoding proteins homologous to effectors of //P. syringae// strains and //X. campestris// | + | XopV was discovered via genome-wide screening for effector candidates in //X. oryzae// pv. //oryzae// (//Xoo//) strain MAFF 311018 in accordance with three criteria: i) ORF encoding proteins homologous to effectors of //P. syringae// strains and //X. campestris// |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | XopV (XOO3803 in Furutani //et al//., 2009) has been shown to be translocated through the T3SS thanks to a XopV fusion with a calmodulin-dependant | + | |
=== Regulation === | === Regulation === | ||
- | Expression of XopV has been shown to be regulated by HrpX using a construction with //xopV// and an adenylate cyclase reporter system in hrp-inducing medium. Also, no adenylate cyclase activity and no //xopV// transcript accumulation could be detected in a //hrpX// deficient strain with the same construction | + | // |
- | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopV//, were significantly reduced in the //Xoo// Δ//xrvC// mutant compared with those in the wild-type strain PXO99< | ||
=== Phenotypes === | === Phenotypes === | ||
- | An individual mutant for //xopV// in //Xoo //PXO99< | + | <font 14px/ |
+ | |||
+ | <font 14px/ | ||
+ | |||
+ | <font 14px/ | ||
- | // | ||
=== Localization === | === Localization === | ||
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Unknown. | Unknown. | ||
- | ===== Conservation ===== | ||
- | |||
- | === In xanthomonads === | ||
- | |||
- | Yes, examples : //X. campestris//, | ||
- | === In other plant pathogens/ | ||
- | |||
- | Yes, examples : //P. cissicola//, | ||
===== Conservation ===== | ===== Conservation ===== | ||
Line 57: | Line 52: | ||
Yes, examples: // | Yes, examples: // | ||
- | ===== Conservation ===== | ||
- | |||
- | === In xanthomonads === | ||
- | |||
- | Yes, examples : //X. campestris//, | ||
- | === In other plant pathogens/ | ||
- | |||
- | Yes, examples : //P. cissicola//, | ||
===== References ===== | ===== References ===== | ||
- | Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the // | + | Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the // |
Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Tsuge S (2009). Identification of novel type III secretion effectors in // | Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Tsuge S (2009). Identification of novel type III secretion effectors in // | ||
- | |||
- | Liu Y, Long J, Shen D, Song C (2016). // | ||
Long J, Song C, Yan F, Zhou J, Zhou H, Yang B (2018). Non-TAL effectors from // | Long J, Song C, Yan F, Zhou J, Zhou H, Yang B (2018). Non-TAL effectors from // | ||
+ | |||
+ | Popov G, Fraiture M, Brunner B, Sessa G (2016). Multiple // | ||
Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ< | Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ< | ||
- | ===== References ===== | + | <font 14px/Arial,Helvetica,sans-serif;; |
- | + | ||
- | Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the // | + | |
- | + | ||
- | Furutani A, Takaoka M, Sanada | + | |
- | + | ||
- | Liu Y, Long J, Shen D, Song C (2016). | + | |
- | + | ||
- | Long J, Song C, Yan F, Zhou J, Zhou H, Yang B (2018). Non-TAL effectors from // | + | |
- | + | ||
- | Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ< | + | |