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bacteria:t3e:xopy

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bacteria:t3e:xopy [2020/07/02 16:09]
rkoebnik [References] 		bacteria:t3e:xopy [2020/07/09 12:13] (current)
rkoebnik
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 ====== XopY ====== ====== XopY ======
  
-Author: Irena Mačionienė\\ +Author: [[https://www.researchgate.net/profile/Irena_Macioniene|Irena Mačionienė]]\\ 
-Internal reviewer: Lucas Morinière\\+Internal reviewer: [[https://www.researchgate.net/profile/Lucas_Moriniere|Lucas Morinière]]\\
 Expert reviewer: FIXME Expert reviewer: FIXME
  
 Class: XopY\\ Class: XopY\\
 Family: XopY\\ Family: XopY\\
-Prototype: XopY, aka Xoo1488 (//Xanthomonas oryzae// pv. //oryzicola// BLS256)\\ +Prototype: XOO1488 (//Xanthomonas oryzae// pv. //oryzae//; strain MAFF 311018)\\ 
-RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/AEQ97580.1|AEQ97580]] (276 aa) +RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/AEQ97580.1|AEQ97580]] (276 aa)\\
 3D structure: Unknown 3D structure: Unknown
  
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 === How discovered? === === How discovered? ===
  
-XopY was discovered by screening the genome of //X. oryzae //pv. //oryzae// MAFF311018 for proteins displaying a N-terminal amino acid pattern associated with T3S substrates in //Pseudomonas syringae// (Furutani //et al.//, 2009). It has been primarily referred to as XOO1488, and then XopY (Song & Yang, 2010).+XopY was discovered by screening the genome of //X. oryzae //pv. //oryzae// MAFF 311018 for proteins displaying a N-terminal amino acid pattern associated with T3S substrates in //Pseudomonas syringae// (Furutani //et al.//, 2009). It has been primarily referred to as XOO1488, and then XopY (Song & Yang, 2010).
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
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 XopY from //X. oryzae //pv. //oryzae// posseses a PIP and ‐10 box in the promoter region (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Yamaguchi //et al//., 2013a). Also, it was shown to be regulated by HrpX (Furutani //et al.//, 2009). XopY from //X. oryzae //pv. //oryzae// posseses a PIP and ‐10 box in the promoter region (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Yamaguchi //et al//., 2013a). Also, it was shown to be regulated by HrpX (Furutani //et al.//, 2009).
  
-qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopY//, were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//XrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup>  (Liu //et al.//, 2016).+qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopY//, were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup>  (Liu //et al.//, 2016).
 === Phenotypes === === Phenotypes ===
  
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 XopY was demonstrated to target OsRLCK185. Expression of XopY in rice cells compromises OsRLCK185-mediated immune responses, which is consistent with the fact that Xoo1488 inhibits trans-phosphorylation of the activation domain of OsRLCK185 by OsCERK1. Interestingly, XopY is phosphorylated by OsRLCK185, suggesting that modification of XopY in host cell may affect their virulent activity (Yamaguchi //et al//., 2013b). XopY was demonstrated to target OsRLCK185. Expression of XopY in rice cells compromises OsRLCK185-mediated immune responses, which is consistent with the fact that Xoo1488 inhibits trans-phosphorylation of the activation domain of OsRLCK185 by OsCERK1. Interestingly, XopY is phosphorylated by OsRLCK185, suggesting that modification of XopY in host cell may affect their virulent activity (Yamaguchi //et al//., 2013b).
- 
 ===== Conservation ===== ===== Conservation =====
  
bacteria/t3e/xopy.1593698953.txt.gz · Last modified: 2020/07/02 16:09 by rkoebnik

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