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- | ====== XopE1 ====== | ||
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- | Author: [[https:// | ||
- | Internal reviewer: [[https:// | ||
- | Expert reviewer: FIXME | ||
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- | Class: XopE\\ | ||
- | Family: XopE1\\ | ||
- | Prototype: XCV0294 (// | ||
- | RefSeq ID: [[https:// | ||
- | Synonym: AvrXacE1 (// | ||
- | 3D structure: Myristoylation motif at their extreme N-terminus (Thieme //et al.//, 2007). | ||
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- | ===== Biological function ===== | ||
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- | === How discovered? === | ||
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- | XopE1 was first identified by sequence homology searches (da Silva //et al//., 2002; Thieme //et al//., 2005). | ||
- | === (Experimental) evidence for being a T3E === | ||
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- | XopE1 fused to the AvrBs3 reporter was shown to be secreted into culture supernatants in a // | ||
- | === Regulation === | ||
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- | Using RT-PCR analyses, XopE1 from //X. euvesicatoria// | ||
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- | Transcriptome analysis (RNA-seq) and qRT-PCR revealed that // | ||
- | === Phenotypes === | ||
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- | // | ||
- | === Localization === | ||
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- | XopE1 fused to GFP reporter in a binary vector under control of the Cauliflower mosaic virus 35S promoter and transiently expressed in //Nicotiana benthamiana// | ||
- | === Enzymatic function === | ||
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- | XopE1 belongs to the HopX effector family, members of which belong to the transglutaminase superfamily (Nimchuk //et al//., 2007). | ||
- | === Interaction partners === | ||
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- | XopE1 was found to physically interact with tomato 14-3-3s (TFT) (Dubrow //et al.//, 2018). In addition, XopE1 was predicted to interact with VirK, which is secreted by the T2SS and for which a possible role in the modulation of plant immune response during the infection process was suggested (Assis //et al//., 2017). | ||
- | ===== Conservation ===== | ||
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- | === In xanthomonads === | ||
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- | Yes (//e.g.//, //X. alfalfa, X. citri, X. euvesicatoria//, | ||
- | === In other plant pathogens/ | ||
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- | Yes (// | ||
- | ===== References ===== | ||
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- | Adlung N, Prochaska H, Thieme S, Banik A, Blüher D, John P, Nagel O, Schulze S, Gantner J, Delker C, Stuttmann J, Bonas U (2016). Non-host resistance induced by the // | ||
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- | Assis RAB, Polloni LC, Patané JSL, Thakur S, Felestrino ÉB, Diaz-Caballero J, Digiampietri LA, Goulart LR, Almeida NF, Nascimento R, Dandekar AM, Zaini PA, Setubal JC, Guttman DS, Moreira LM (2017). Identification and analysis of seven effector protein families with different adaptive and evolutionary histories in plant-associated members of the // | ||
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- | da Silva AC, Ferro JA, Reinach FC, Farah CS, Furlan LR, Quaggio RB, Monteiro-Vitorello CB, Van Sluys MA, Almeida NF, Alves LM, do Amaral AM, Bertolini MC, Camargo LE, Camarotte G, Cannavan F, Cardozo J, Chambergo F, Ciapina LP, Cicarelli RM, Coutinho LL, Cursino-Santos JR, El Dorry H, Faria JB, Ferreira AJ, Ferreira RC, Ferro MI, Formighieri EF, Franco MC, Greggio CC, Gruber A, Katsuyama AM, Kishi LT, Leite RP, Lemos EG, Lemos MV, Locali EC, Machado MA, Madeira AM, Martinez-Rossi NM, Martins EC, Meidanis J, Menck CF, Miyaki CY, Moon DH, Moreira LM, Novo MT, Okura VK, Oliveira, MC, Oliveira VR, Pereira HA, Rossi A, Sena JA, Silva C, de Souza RF, Spinola LA,Takita MA, Tamura RE, Teixeira EC, Tezza RI, Trindade dos SM, Truffi D, Tsai, SM, White FF, Setubal JC, Kitajima JP (2002). Comparison of the genomes of two // | ||
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- | Dubrow Z, Sunitha S, Kim JG, Aakre CD, Girija AM, Sobol G, Teper D, Chen YC, Ozbaki-Yagan N, Vance H, Sessa G, Mudgett MB (2018). Tomato 14-3-3 proteins are required for //Xv3// disease resistance and interact with a subset of // | ||
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- | Nimchuk ZL, Fisher EJ, Desvaux D, Chang JH, Dangl JL (2007). The HopX (AvrPphE) family of // | ||
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- | Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of // | ||
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- | Thieme F, Koebnik R, Bekel T, Berger C, Boch J, Büttner D, Caldana C, Gaigalat L, Goesmann A, Kay S, Kirchner O, Lanz C, Linke B, McHardy AC, Meyer F, Mittenhuber G, Nies DH, Niesbach-Klösgen U, Patschkowski T, Rückert C, Rupp O, Schneiker S, Schuster SC, Vorhölter F, Weber E, Pühler A, Bonas U, Bartels D, Kaiser O (2005). Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium // | ||
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- | Thieme F, Szczesny R, Urban A, Kirchner O, Hause G, Bonas U (2007). New type III effectors from // | ||
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- | Wei C, Ding T, Chang C, Yu C, Li X, Liu Q (2019). Global regulator PhoP is necessary for motility, biofilm formation, exoenzyme production and virulence of // | ||