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bacteria:t3e:xopf [2020/07/08 18:29] rkoebnik [XopF] |
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- | ====== XopF ====== | ||
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- | Author: [[https:// | ||
- | Internal reviewer: [[https:// | ||
- | Expert reviewer: FIXME | ||
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- | Class: XopF\\ | ||
- | Family: XopF1, XopF2, XopF3\\ | ||
- | Prototype: XopF (// | ||
- | RefSeq ID: XopF1 [[https:// | ||
- | 3D structure: Unknown | ||
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- | ===== Biological function ===== | ||
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- | === How discovered? === | ||
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- | XopF1 and XopF2 were identified in a genetic screen, using a Tn// | ||
- | === (Experimental) evidence for being a T3E === | ||
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- | Type III-dependent secretion of XopF1 and XopF2 was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004). | ||
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- | Fragments of the //xopF1// gene are located within the //hrp// cluster of many // | ||
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- | XopF1 belongs to the class A effectors (Büttner //et al//., 2006). XopF2 is 59% identical and 68% similar to XopF1 when analysed with the pairwise BLAST algorithm. //xopF2// appears to be co-transcribed with ORF1. ORF1 analysis revealed characteristics shared by type III chaperones, and is suggested to encode an Xcv chaperone (Roden //et al//., 2004). | ||
- | === Regulation === | ||
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- | RT-PCR analysis revealed //xopF1// is regulated by //hrpG// and //hrpX// and that //xopF1//, //hpaD//, //hpaI// belong to the same operon. Upstream there is a PIP box which provides binding site for HrpX (Büttner //et al//., 2007). | ||
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- | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopF//, were significantly reduced in the // | ||
- | === Phenotypes === | ||
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- | * Roden et al. did not find significant growth defects of a // | ||
- | * To study the possible virulence function of the putative // | ||
- | * Later, // | ||
- | * Additionally, | ||
- | * // | ||
- | === Localization === | ||
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- | XopF2 localizes in the Golgi apparatus, while XopF1 has been found in cytoplasm (Popov //et al//., 2016) and plasma membrane (Mondal //et al//., 2016). XopF1 is encoded within // | ||
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- | === Enzymatic function === | ||
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- | Unknown. | ||
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- | === Interaction partners === | ||
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- | XopF1 secretion and translocation is T3SS-dependent; | ||
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- | ===== Conservation ===== | ||
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- | === In xanthomonads === | ||
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- | Yes (//e.g.//, //X. arboricola, X. bromi//, //X. citri, X. oryzae//, //X. euvesicatoria//, | ||
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- | === In other plant pathogens/ | ||
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- | Unknown. | ||
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- | ===== References ===== | ||
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- | Büttner D, Lorenz C, Weber E, Bonas U (2006). Targeting of two effector protein classes to the type III secretion system by a HpaC- and HpaB-dependent protein complex from // | ||
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- | Büttner D, Noël L, Stuttmann J, Bonas U (2007). Characterization of the nonconserved // | ||
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- | Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in // | ||
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- | Liu Y, Long J, Shen D, Song C (2016). // | ||
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- | Mondal K K, Verma G, Manju, Junaid A, Mani C (2016). Rice pathogen // | ||
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- | Popov G, Fraiture M, Brunner F, Sessa G (2016). Multiple // | ||
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- | Roden J, Belt B, Ross J, Tachibana T, Vargas J, Mudgett M (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // | ||
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- | ===== Further reading ===== | ||
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- | Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of Xanthomonas campestris pv. // | ||