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bacteria:t3e:xopz [2020/08/02 22:49] jfpothier |
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- | ====== XopZ ====== | ||
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- | Author: Marlène Lachaux\\ | ||
- | Internal reviewer: [[https:// | ||
- | Expert reviewer: FIXME | ||
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- | Class: XopZ\\ | ||
- | Family: XopZ\\ | ||
- | Prototype: XopZ (// | ||
- | RefSeq ID: [[https:// | ||
- | 3D structure: Unknown. The N-terminus of XopZ< | ||
- | ===== Biological function ===== | ||
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- | === How discovered? === | ||
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- | The first mention of XopZ as an homolog of HopAS1 in// Xanthomonas oryzae// MAFF311018 was made by Furutani //et al//. (2009). Indeed, the locustag XOO2402 ([[https:// | ||
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- | In 2009, the generation of mutants for 18 non-TAL type 3 effector genes in //Xoo// strain PXO99< | ||
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- | XopZ2 was described in Potnis //et al//., 2011 as a novel candidate effector gene upstream of hrpW in // | ||
- | === (Experimental) evidence for being a T3E === | ||
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- | The secretion of XopZ //in planta// was shown using a //B. pertussis// Cya translocation reporter assay (Furutani //et al//., 2009). With a PIP box 58 bp upstream of the predicted translation start site, // | ||
- | === Regulation === | ||
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- | The //xopZ// gene was shown to be expressed in a // | ||
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- | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopZ//, were significantly reduced in the // | ||
- | === Phenotypes === | ||
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- | PXO99< | ||
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- | A role of XopZ in full virulence was also clearly shown in // | ||
- | === Localization === | ||
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- | XopZ< | ||
- | === Enzymatic function === | ||
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- | XopZ< | ||
- | === Interaction partners === | ||
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- | XopZ interacts with a putative host E3 ubiquitin ligase protein PBP (s-ribonuclease) //in vitro// and //in vivo//. Regions containing 193 aa - 225 aa of PBP is required for interacting with XopZ. PBP is a negative regulator of host immune response based on the disease phenotype in PBP-knockout rice plants. C1A directly interacts and strongly degrades PBP through its cysteine protease activity, leading to a homeostatic state of PBP in plant cells (Zhou //et al//., 2015). | ||
- | ===== Conservation ===== | ||
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- | === In xanthomonads === | ||
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- | Yes, found to be conserved in all // | ||
- | === In other plant pathogens/ | ||
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- | Related genes are also found in several // | ||
- | ===== References ===== | ||
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- | Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in // | ||
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- | Liu Y, Long J, Shen D, Song C (2016). // | ||
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- | Lindeberg M, Stavrinides J, Chang JH, Alfano JR, Collmer A, Dangl JL, Greenberg JT, Mansfield JW, Guttman DS (2005). Proposed guidelines for a unified nomenclature and phylogenetic analysis of type III Hop effector proteins in the plant pathogen // | ||
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- | Long J, Song C, Yan F, Zhou J, Zhou H, Yang B (2018). Non-TAL effectors from // | ||
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- | Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // | ||
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- | Potnis N, Krasileva K, Chow V, Almeida NF, Patil PB, Ryan RP, Sharlach M, Behlau F, Dow JM, Momol M, White FF, Preston JF, Vinatzer BA, Koebnik R, Setubal JC, Norman DJ, Staskawicz BJ, Jones JB (2011). Comparative genomics reveals diversity among xanthomonads infecting tomato and pepper. BMC Genomics 12: 146. DOI: [[https:// | ||
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- | Ryan RP, Koebnik R, Szurek B, Boureau T, Bernal A, Bogdanove A, Dow JM (2009). Passing GO (gene ontology) in plant pathogen biology: a report from the // | ||
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- | Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of // | ||
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- | Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ < | ||
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- | Zhou J (2015). Host target genes of the // | ||