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bacteria:t3e:xopb [2020/04/10 13:09] jfpothier |
bacteria:t3e:xopb [2020/07/08 18:19] (current) rkoebnik [XopB] |
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====== XopB ====== | ====== XopB ====== | ||
- | Author: Ralf Koebnik\\ | + | Author: |
- | Reviewer: FIXME\\ | + | Internal reviewer: [[https:// |
Expert reviewer: FIXME | Expert reviewer: FIXME | ||
Class: XopB\\ | Class: XopB\\ | ||
Family: XopB\\ | Family: XopB\\ | ||
- | Prototype: XopB (// | + | Prototype: XopB (// |
RefSeq ID: [[https:// | RefSeq ID: [[https:// | ||
3D structure: Unknown | 3D structure: Unknown | ||
Line 14: | Line 14: | ||
=== How discovered? === | === How discovered? === | ||
- | XopB was discovered in a cDNA-AFLP screen.< | ||
- | // | + | XopB was discovered in a cDNA-AFLP screen |
+ | === (Experimental) evidence | ||
- | //Regulation:// The //xopB// gene was shown to be expressed | + | A chimeric protein consisting of a C-terminally truncated XopB where the last 52 residues (5 kDa) were replaced by the triple c-myc epitope (5 kDa) was secreted into culture supernatants of a strain with a constitutively active form of //hrpG// in a type III secretion-dependent manner (Noël |
+ | === Regulation === | ||
- | //Phenotypes:// A deletion of //xopB// did not affect pathogenicity or bacterial growth in plants.<sup>[1]</sup> Later it was found that XopB contributes to disease symptoms and bacterial growth.<sup>[3,6]</sup> Infection of susceptible pepper plants with a strain lacking | + | The //xopB// gene was shown to be expressed in a //hrpG//- and // |
+ | === Phenotypes === | ||
- | When expressed in yeast, XopB attenuated cell proliferation.< | + | A deletion of //xopB// did not affect pathogenicity or bacterial growth in plants (Noël //et al.//, 2001). Later it was found that XopB contributes to disease symptoms and bacterial growth (Schulze //et al.//, 2012; Priller //et al.//, 2016). Infection of susceptible pepper plants with a strain lacking //xopB// resulted in increased formation of salicylic acid (SA) and expression of pathogenesis-related (PR) genes (Priller //et al.//, 2016). |
+ | === Localization === | ||
- | // | + | XopB localizes to the Golgi apparatus and cytoplasm of the plant cell and interferes with eukaryotic vesicle trafficking |
+ | === Enzymatic function === | ||
- | //Enzymatic function:// | + | Unknown. |
- | //Interaction partners:// Unknown | + | === Interaction partners |
- | **Conservation** | + | Unknown. |
- | //In xanthomonads:// | + | ===== Conservation ===== |
- | //In other plant pathogens/ | + | === In xanthomonads === |
- | **References** | + | Yes (e.g., //X. fragariae//, |
+ | === In other plant pathogens/ | ||
- | - Noël L, Thieme F, Nennstiel D, Bonas U (2001). cDNA-AFLP analysis unravels a genome-wide // | + | Yes (e.g., //Pseudomonas// spp., //Ralstonia solanacearum//, //Acidovorax// spp., //Pantoea agglomerans//) (Schulze |
- | - Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of //Xanthomonas campestris// pv. // | + | ===== References ===== |
- | - Schulze S, Kay S, Büttner D, Egler M, Eschen-Lippold L, Hause G, Krüger A, Lee J, Müller O, Scheel D, Szczesny R, Thieme F, Bonas U (2012). Analysis of new type III effectors from //Xanthomonas// uncovers XopB and XopS as suppressors of plant immunity. New Phytol. 195(4): 894-911. doi: | + | |
- | - Sonnewald S, Priller JP, Schuster J, Glickmann E, Hajirezaei MR, Siebig S, Mudgett MB, Sonnewald U (2012). Regulation of cell wall-bound invertase in pepper leaves by //Xanthomonas campestris// pv. // | + | |
- | - Harrison J, Studholme DJ (2014). Draft genome sequence of //Xanthomonas axonopodis// | + | |
- | - Priller JP, Reid S, Konein P, Dietrich P, Sonnewald S (2016). The // | + | |
- | - Prochaska H, Thieme S, Daum S, Grau J, Schmidtke C, Hallensleben M, John P, Bacia K, Bonas U (2018). A conserved motif promotes HpaB-regulated export of type III effectors from // | + | |
+ | Harrison J, Studholme DJ (2014). Draft genome sequence of // | ||
+ | |||
+ | Noël L, Thieme F, Nennstiel D, Bonas U (2001). cDNA-AFLP analysis unravels a genome-wide // | ||
+ | |||
+ | Priller JPR, Reid S, Konein P, Dietrich P, Sonnewald S (2016). The // | ||
+ | |||
+ | Prochaska H, Thieme S, Daum S, Grau J, Schmidtke C, Hallensleben M, John P, Bacia K, Bonas U (2018). A conserved motif promotes HpaB-regulated export of type III effectors from // | ||
+ | |||
+ | Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of // | ||
+ | |||
+ | Schulze S, Kay S, Büttner D, Egler M, Eschen-Lippold L, Hause G, Krüger A, Lee J, Müller O, Scheel D, Szczesny R, Thieme F, Bonas U (2012). Analysis of new type III effectors from // | ||
+ | |||
+ | Sonnewald S, Priller JPR, Schuster J, Glickmann E, Hajirezaei MR, Siebig S, Mudgett MB, Sonnewald U (2012). Regulation of cell wall-bound invertase in pepper leaves by // | ||