This shows you the differences between two versions of the page.
Both sides previous revision Previous revision Next revision | Previous revision | ||
bacteria:t3e:xopi [2020/07/01 10:33] rkoebnik [References] |
bacteria:t3e:xopi [2020/07/09 10:16] (current) rkoebnik |
||
---|---|---|---|
Line 1: | Line 1: | ||
====== XopI ====== | ====== XopI ====== | ||
- | Author: Trainees from the EuroXanth | + | Author: |
Internal reviewer: Isabel Rodrigues\\ | Internal reviewer: Isabel Rodrigues\\ | ||
Expert reviewer: FIXME | Expert reviewer: FIXME | ||
Class: XopI\\ | Class: XopI\\ | ||
- | Family: | + | Family: |
- | Prototype: (// | + | Prototype: (// |
RefSeq ID: [[https:// | RefSeq ID: [[https:// | ||
3D structure: Unknown | 3D structure: Unknown | ||
Line 15: | Line 15: | ||
=== How discovered? === | === How discovered? === | ||
- | Effector proteins (T3Es) can suppress the plant innate immunity and alter the plant metabolism to the pathogen’s advantage. The T3E XopI was identified in Xcv strain 85-10 due to a F-box motif based on the presence of a PIP (pathogen-inducible promoter) box in its promoter region. XopI secretion and translocation was shown during the interaction of Xcv with resistant pepper plants (Schulze //et al//., 2012). Moreover, interaction studies in yeast showed that XopI specifically interacts with one out of 21 // | + | Effector proteins (T3Es) can suppress the plant innate immunity and alter the plant metabolism to the pathogen’s advantage. The T3E XopI was identified in //Xcv// strain 85-10 due to a F-box motif based on the presence of a PIP (pathogen-inducible promoter) box in its promoter region. XopI secretion and translocation was shown during the interaction of //Xcv// with resistant pepper plants (Schulze //et al//., 2012). Moreover, interaction studies in yeast showed that XopI specifically interacts with one out of 21 // |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | The transcripts of XopI were amplified from Xcv derivative 85* strain, which expresses a constitutively active HrpG point mutant resulting in constitutive expression of the T3S system, suggesting co‐expression with T3S genes (Schulze //et al//., 2012). To investigate whether //xopI// was indeed T3SS dependently secreted and translocated into the plant cell, a translational fusion with the reporter protein | + | The transcripts of XopI were amplified from //Xcv// derivative 85* strain, which expresses a constitutively active HrpG point mutant resulting in constitutive expression of the T3S system, suggesting co‐expression with T3S genes (Schulze //et al//., 2012). To investigate whether //xopI// was indeed T3SS dependently secreted and translocated into the plant cell, a translational fusion with the reporter protein |
+ | |||
+ | Translocation class; classification based on HpaB dependence (Büttner //et al.//, 2006). | ||
=== Regulation === | === Regulation === | ||
- | XopI is presumably controlled by both HrpG and HrpX. The HrpX-dependent induction of //xopR// has been described previously (Koebnik //et al.//, 2006). HrpG‐ and HrpX‐dependent co‐regulation with the T3S system | + | XopI is presumably controlled by both HrpG and HrpX. The HrpX-dependent induction of //xopR// has been described previously (Koebnik //et al.//, 2006). HrpG‐ and HrpX‐dependent co‐regulation with the T3S system. |
=== Phenotypes === | === Phenotypes === | ||
Line 57: | Line 59: | ||
Üstün S, Börnke F (2014). Interactions of // | Üstün S, Börnke F (2014). Interactions of // | ||
+ | |||
+ | ===== Further reading ===== | ||
+ | |||
+ | Thieme F (2006). Genombasierte Identifizierung neuer potentieller Virulenzfaktoren von // | ||