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Author: Doron Teper
Reviewer:

Class: XopJ
Family: XopJ3
Prototype: XCV0471 ( CKGE_TMP_i Xanthomonas CKGE_TMP_i CKGE_TMP_i euvesicatoria CKGE_TMP_i )
RefSeq ID: CAJ22102
Synonym: AvrRxv
3D structure: Unknown

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Biological function</font> <font 12.0pt/inherit;;inherit;;inherit>How discovered?</font> <font 12.0pt/inherit;;inherit;;inherit>AvrRxv/XopJ3 of CKGE_TMP_i X. euvesicatoria CKGE_TMP_i was identified to promote HR in non-host bean plants (Whalen et al. 1988).

(Experimental) evidence for being a T3E</font> <font 12.0pt/inherit;;inherit;;inherit>Tn5 insertions in multiple CKGE_TMP_i hrp CKGE_TMP_i genes in XcvT 75-3 eliminated AvrRxv/XopJ3-mediated HR in tomato line Hawaii 7998 (Ciesiolka et al. 1999). Disruption of the type III secretion system in CKGE_TMP_i Xcv CKGE_TMP_i race T3 abolished secretion of AvrRxv into culture media (Bonshtien et al. 2004).

Regulation</font> <font 12.0pt/inherit;;inherit;;inherit>CKGE_TMP_i avrRxv is constitutively expressed in CKGE_TMP_i Xanthomonas CKGE_TMP_i tomato strain XcvT 92-14 (Ciesiolka et al. 1999). The expression of CKGE_TMP_i avrRxv CKGE_TMP_i was similar in NYB and XVM2 and was not dependent on CKGE_TMP_i hrpG CKGE_TMP_i or CKGE_TMP_i hrpX CKGE_TMP_i (Ciesiolka et al 1999). CKGE_TMP_i avrRxv CKGE_TMP_i has a perfect PIP-box starting at bp 465 with the sequence TTCGC-N15-TTCGC (Ciesiolka et al 1999). Phenotypes</font> <font 12.0pt/inherit;;inherit;;inherit>CKGE_TMP_i avrRxv induce hypersensitive response in tomato line Hawaii 7998 (Whalen et al. 1993)( Bonshtien et al. 2004), bean (Whalen et al. 1988) and CKGE_TMP_i Nicotiana benthamiana CKGE_TMP_i (Albers et al. 2019) CKGE_TMP_i . CKGE_TMP_i Inactivation of CKGE_TMP_i arvRxv CKGE_TMP_i ( CKGE_TMP_i avrRxv CKGE_TMP_i :: Ω ) in XcvT 75-3 eliminated HR induction and promoted growth in tomato line Hawaii 7998 (Whalen et al. 1993). CKGE_TMP_i Xcv CKGE_TMP_i race T3 ( CKGE_TMP_i X. perforans CKGE_TMP_i ) introduced with CKGE_TMP_i avrRxv CKGE_TMP_i induced resistance response in tomato line Hawaii 7998 which was characterized by HR, reduced bacterial growth CKGE_TMP_i in planta CKGE_TMP_i and significant alteration in host transcriptional profile (Bonshtien et al. 2004).

Localization</font> <font 12.0pt/inherit;;inherit;;inherit>AvrRxv is localized predominantly to the cytoplasm and is possibly associated with the plasma and nuclear membranes (Bonshtien et al. 2004).

Enzymatic function</font> <font 12.0pt/inherit;;inherit;;inherit>AvrRxv/XopJ3 belongs to the group of YopJ-family effectors. YopJ-family effectors share a conserved catalytic triad that is identical to that of the C55 family of cysteine proteases (Ma and Ma, 2016). Mutations in the AvrRxv protease catalytic core impaired elicitation of HR and inhibition of bacterial growth in tomato line Hawaii 7998 (Bonshtien et al. 2004).

Interaction partners</font> <font 12.0pt/inherit;;inherit;;inherit>AvrRxv interacts with tomato 14-3-3 protein (Whalen et al. 2008).

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Conservation</font> <font 12.0pt/inherit;;inherit;;inherit>In xanthomonads</font> <font 12.0pt/inherit;;inherit;;inherit>Yes ( CKGE_TMP_i X. euvesicatoria, X. hortorum , X. nasturtii, X. axonopodis CKGE_TMP_i )

In other plant pathogens/symbionts</font> <font 11pt/inherit;;inherit;;inherit>Yes (Pseudomonas CKGE_TMP_i syringe CKGE_TMP_i spp. (HopZ-family), Ralstonia solanacearum (PopP1), Acidovorax citrulli, Bradyrhizobium sp., Mesorhizobium sp., Sinorhizobium fredii)

References